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http://dx.doi.org/10.17576/jsm-2018-4712-18
Current Progress in Production of Flavonoids
using Systems and Synthetic Biology Platforms
(Kajian Semasa dalam Penghasilan Flavonoid
menggunakan Teknik Biologi Sistem dan Biologi Sintetik)
KU NURUL AQMAR KU BAHAUDIN1, SURIANA SABRI2,3, AHMAD BAZLI RAMZI1, ADAM LEOW THEAN CHOR2,4, TEWIN TENCOMNAO5 & SYARUL NATAQAIN BAHARUM1*
1Institute of Systems Biology (INBIOSIS), Universiti
Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia
2Enzyme and Microbial Technology Research
Center, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra
Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia
3Department of Microbiology, Faculty of
Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400
Serdang, Selangor Darul Ehsan, Malaysia
4Department of Cell and Molecular Biology, Faculty of
Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400
Serdang, Selangor Darul Ehsan, Malaysia
5Department of Clinical Chemistry, Faculty of Allied Health
Sciences, Chulalongkorn University, Bangkok, Thailand
Received: 30 May 2018/Accepted: 14 September
2018
ABSTRACT
Flavonoid is an
industrially-important compound due to its high pharmaceutical and
cosmeceutical values. However, conventional methods in extracting and
synthesizing flavonoids are costly, laborious and not sustainable due to small
amount of natural flavonoids, large amounts of chemicals and space used.
Biotechnological production of flavonoids represents a viable and sustainable
route especially through the use of metabolic engineering strategies in
microbial production hosts. In this review, we will highlight recent strategies
for the improving the production of flavonoids using synthetic biology
approaches in particular the innovative strategies of genetically-encoded
biosensors for in vivo metabolite analysis and high-throughput screening
methods using fluorescence-activated cell sorting (FACS).
Implementation of transcription factor based-biosensor for microbial flavonoid
production and integration of systems and synthetic biology approaches for
natural product development will also be discussed.
Keywords: Biosensor; flavonoid;
metabolic engineering; microbial systems; synthetic biology
ABSTRAK
Flavonoid merupakan sebatian penting
secara industri disebabkan oleh permintaannya yang tinggi dalam
farmaseutik dan mempunyai nilai kosmeseutik. Walau bagaimanapun,
kebiasaannya kaedah pengekstrakan dan sintesis flavonoid adalah
berkos tinggi, rumit dan tidak mapan disebabkan oleh penghasilan
flavonoid semula jadi yang rendah. Selain itu, kaedah ini juga menggunakan
ruang makmal yang besar dan bahan kimia dalam kuantiti yang banyak.
Hasil pengeluaran bioteknologi daripada flavonoid menunjukkan berdaya
maju dan laluan yang mapan. Ini dapat dilihat melalui pelbagai kaedah
yang digunakan di dalam kejuruteraan metabolik dan biologi sintetik
dengan menggunakan mikrob sebagai hos bagi pengeluaran hasil produk.
Di dalam ulasan kajian ini, kami akan mengetengahkan kaedah bagi
meningkatkan penghasilan flavonoid menggunakan teknik biologi sintetik.
Melalui kaedah ini, teknik biosensor berasaskan pengekod genetik
yang digunakan bagi menganalisis metabolit secara in vivo dan kaedah saringan daya pemprosesan tinggi berasaskan
pengisihan sel teraktif berpendarfluor (FACS). Oleh yang demikian, penggunaan
bioteknologi terhadap penghasilan flavonoid pada masa kini menggunakan
pendekatan integrasi antara biologi sistem dan biologi sintetik
bagi perkembangan produk semula jadi akan dibincangkan dengan lebih
lanjut.
Kata
kunci: Biologi sintetik; biosensor; flavonoid; kejuruteraan metabolisme;
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*Corresponding author; email: nataqain@ukm.edu.my
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