Sains Malaysiana 49(6)(2020): 1245-1272
http://dx.doi.org/10.17576/jsm-2020-4906-04
Identification
and Analysis of microRNAs Responsive to Abscisic Acid and Methyl Jasmonate
Treatments in Persicaria minor
(Pengenalpastian dan Analisis Gerak Balas mikroRNA kepada
Rawatan Asid Absisik dan Metil Jasmonat dalam Persicaria
minor)
ABDUL
FATAH A. SAMAD1,2, NAZARUDDIN NAZARUDDIN3, JAEYRES JANI3 & ISMANIZAN ISMAIL1,4*
1Institute of Systems Biology, Universiti
Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia
2Department of Biosciences, Faculty of
Science, Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor Darul Takzim, Malaysia
3Department of Chemistry, Faculty of
Mathematics and Natural Sciences, Syiah Kuala University
Darussalam,
23111 Banda Aceh, Indonesia
4Centre for Biotechnology and
Functional Food, Faculty of Science and Technology, Universiti Kebangsaan
Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia
Received:
9 August 2019/Accepted: 14 February 2020
ABSTRACT
Persicaria minor has been
recognised as a plant with high content of volatile organic compounds (VOC)
especially terpenoid and green leaf volatile (GLV). Previous finding had showed
signaling molecules such as abscisic acid (ABA) and methyl jasmonate (MeJA) can
increase the VOC content in plant. In this study, we performed next generation
sequencing (NGS) of small RNA to uncover miRNAs roles and their response to
both phytohormones (ABA and MeJA) in P.
minor. For both ABA and MeJA treated P. minor, small RNA libraries containing 17,253,566 and 40,437,576
reads were generated, respectively. In addition, 18,634,904 reads were
generated in plant treated with sterile distilled water which served as
control. In these libraries, a total of 88 miRNAs were identified, comprising
41 known and 47 novel miRNAs. It was observed that 21 and 38 miRNAs were
significantly regulated in ABA and MeJA libraries, respectively. Four selected
miRNAs related to VOC pathways were subjected to RT-qPCR analysis and found to
display diverse expression patterns with their targets. This study provides the
initial framework for further exploration of miRNA roles in ABA and MeJA
responses.
Keywords:
Abscisic acid; methyl jasmonate; microRNA; Persicaria minor; volatile organic compound
ABSTRAK
Persicaria minor telah dikenal pasti sebagai tumbuhan yang
mempunyai kandungan sebatian organik meruap (VOC) yang tinggi terutama
terpenoid dan sebatian daun hijau meruap (GLV). Kajian lepas menunjukkan
molekul pengisyaratan seperti asid absisik (ABA) dan metil jasmonat (MeJA)
boleh meningkatkan kandungan VOC dalam tumbuhan. Dalam kajian ini, kami menjalankan penjujukan generasi terkini (NGS)
RNA kecil untuk merungkai peranan miRNA dan tindak balasnya terhadap kedua-dua
fitohormon (ABA dan MeJA) dalam P.
minor. Bagi kedua-dua rawatan ABA dan MeJA terhadap P. minor, perpustakaan kecil RNA masing-masing telah menjana sejumlah
17,253,566 dan 40,437,576 bacaan. Tambahan lagi, sejumlah 18,634,904 bacaan
telah dijana daripada tumbuhan terawat air suling steril yang bertindak sebagai
kawalan. Dalam perpustakaan tersebut, sejumlah 88 miRNA telah dikenal pasti
yang terdiri daripada 41 miRNA yang telah diketahui fungsinya dan 47 miRNA
novel. Sejumlah 21 dan 38 miRNA masing-masing telah dicerap dikawal atur secara
signifikan dalam perpustakaan ABA dan MeJA. Sebanyak empat miRNA yang berkait
dengan tapak jalan VOC telah dikaji melalui analisis RT-qPCR dan didapati
menunjukkan corak pengekspresan yang pelbagai terhadap transkrip sasaran
masing-masing. Kajian ini menyediakan rangka kerja awal untuk penerokaan
selanjutnya mengenai peranan miRNA dalam tindak balas ABA dan MeJA.
Kata
kunci: Asid absisik; metil jasmonat; mikroRNA; Persicaria minor; sebatian organik meruap
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*Corresponding author; email: maniz@ukm.edu.my
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